制限酵素
Assume we are cloning a 1.5 kilobase fragment generated by cleavage of genomic DNA with BamH1 restriction enzyme into a plasmid which has a single site where it can be cleaved with the same restriction enzyme.
The original plasmid is 5 kilobase in length. Following insertion of the plasmid and treastment with DNA ligase, you exmaine the size of your consruct electrophoretically. To you surprise, you find plasmids with sizes of 5, 7.5, 9, 10, 12, and 15 kilobase.
Explain how these results might have been obtained? Can you think of any way in which the problem might have been prevented?
この問題がテストで出たんですがよくわかりませんでした。
この問題は要するに、元々のプラスミドにBamH1で切られるサイトが何個あって、その場所はどこかってことを聞いてるんですか？
お礼
ありがとうございます。 微妙ですが、使い分ける価値がありますね。