Protein dissociation from and binding to DNA were studied using a constant force
Binding of single protein to DNA decreases the DNA length by 3 nm
The DNA is extended to its contour length when pulled by both ends
物理での英語がよく分からないので質問させてください。
物理での英語がよく分からないので質問させてください。
Protein dissociation from and binding to DNA were carried out by applying a constant force and monitoring the DNA extension. Binding of single protein to DNA decreases the DNA end-to-end contour length by 3 nm. At equilibrium, the protein is bound to DNA. After the system was allowed to reach equilibrium, constant force was applied to DNA (two ends were pulled) and the DNA extension were measured.
You can assume that the DNA is extended to its contour length and that for the sake of simplicity, under selected conditions you can ignore the DNA elasticity.
文章中の"DNA is extended to its contour length"というのがどういう意味なのかよく分かりません。これは「DNAが伸び得る最大まで伸びる」という解釈でいいのでしょうか？つまりDNAの両端を引っ張ると3nm伸びるという事で合ってるのでしょうか？
