生物科学の英文です。翻訳お願いします
Transcription factors are important for the generation and fate determination
of various cell types6–8.
It has been hypothesized that some
transcription factors may regulate the differentiation of TRCs, which
are continually self-renewing even in adult animals.
We conducted a
genome-wide comparative survey of gene expression profiles from isolated
taste buds, circumvallate epithelium and non-gustatory
tongue
epithelium (Supplementary Methods) and found that mRNA for Skn-1
(also known as Pou2f3), originally identified in epidermal keratinocytes9,10
as Skn-1a/i, which encodes two functionally distinct POU
homeodomain proteins, Skn-1a and Skn-1i, is expressed in a subset
of taste bud cells (Fig. 1a).
Double fluorescence in situ hybridization
analysis revealed that Skn-1a/i mRNA was expressed in T1r3 (also
known as Tas1r3)-positive sweet and umami TRCs, T2r5 (also known
as Tas2r105)-positive bitter TRCs, and a small population of oval cells in
the basal region of the taste buds, but not in Pkd2l1-positive sour TRCs
and NTPDase2 (also known as Entpd2)-positive cells with unknown
functions (Fig. 1b).
A detailed analysis using specific probes for Skn-1a
and Skn-1i (probes a-2 and i, respectively, in Supplementary Fig. 1a)
revealed that only Skn-1a mRNA was expressed (Supplementary
Fig. 1b), and the 5-kb upstream region of the mouse Skn-1a gene efficiently
induced GFP expression in Skn-1a/i–positive TRCs in transgenic mice (Supplementary Fig. 1c).
These results indicate that Skn-1a is the
major transcript of the Skn-1 locus in sweet, umami and bitter TRCs, as
well as in basal cells in the taste buds.
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