コンディショナルノックアウト法について

質問

コンディショナルノックアウト法の概要と方法を詳しく教えてください。

回答No.1

MIYD

詳しく知りたいのですね。
まずは、下記の論文とそれらに引用されている論文を読んでみましょう。
1: Related Articles, LinksSzymańska H.

[Genetically engineered mice: mouse models for cancer research]
Postepy Hig Med Dosw (Online). 2007 Oct 26;61:639-45. Review. Polish.
PMID: 17971765 [PubMed - indexed for MEDLINE]2: Related Articles, LinksChecler F, Sunyach C, Pardossi-Piquard R, Sévalle J, Vincent B, Kawarai T, Girardot N, St George-Hyslop P, da Costa CA.

The gamma/epsilon-secretase-derived APP intracellular domain fragments regulate p53.
Curr Alzheimer Res. 2007 Sep;4(4):423-6. Review.
PMID: 17908046 [PubMed - indexed for MEDLINE]3: Related Articles, LinksFriedman LM, Dror AA, Avraham KB.

Mouse models to study inner ear development and hereditary hearing loss.
Int J Dev Biol. 2007;51(6-7):609-31. Review.
PMID: 17891721 [PubMed - indexed for MEDLINE]4: Related ArticlesWintermantel TM, Elzer J, Herbison AE, Fritzemeier KH, Schütz G.

Genetic dissection of estrogen receptor signaling in vivo.
Ernst Schering Found Symp Proc. 2006;(1):25-44. Review.
PMID: 17824170 [PubMed - indexed for MEDLINE]5: Related Articles, LinksTian Y, James S, Zuo J, Fritzsch B, Beisel KW.

Conditional and inducible gene recombineering in the mouse inner ear.
Brain Res. 2006 May 26;1091(1):243-54. Epub 2006 Feb 20. Review.
PMID: 16488403 [PubMed - indexed for MEDLINE]6: Related Articles, LinksZenz R, Wagner EF.

Jun signalling in the epidermis: From developmental defects to psoriasis and skin tumors.
Int J Biochem Cell Biol. 2006;38(7):1043-9. Epub 2005 Dec 20. Review.
PMID: 16423552 [PubMed - indexed for MEDLINE]7: Related Articles, LinksKeller C, Capecchi MR.

New genetic tactics to model alveolar rhabdomyosarcoma in the mouse.
Cancer Res. 2005 Sep 1;65(17):7530-2. Review.
PMID: 16140913 [PubMed - indexed for MEDLINE]8: Related Articles, LinksReuter H, Pott C, Goldhaber JI, Henderson SA, Philipson KD, Schwinger RH.

Na(+)--Ca2+ exchange in the regulation of cardiac excitation-contraction coupling.
Cardiovasc Res. 2005 Aug 1;67(2):198-207. Review.
PMID: 15935336 [PubMed - indexed for MEDLINE]9: Related Articles, LinksHaase VH.

The VHL tumor suppressor in development and disease: functional studies in mice by conditional gene targeting.
Semin Cell Dev Biol. 2005 Aug-Oct;16(4-5):564-74. Epub 2005 Apr 26. Review.
PMID: 15908240 [PubMed - indexed for MEDLINE]10: Related Articles, LinksKasper S.

Survey of genetically engineered mouse models for prostate cancer: analyzing the molecular basis of prostate cancer development, progression, and metastasis.
J Cell Biochem. 2005 Feb 1;94(2):279-97. Review.
PMID: 15565647 [PubMed - indexed for MEDLINE]11: Related Articles, LinksCollinson JM, Hill RE, West JD.

Analysis of mouse eye development with chimeras and mosaics.
Int J Dev Biol. 2004;48(8-9):793-804. Review.
PMID: 15558472 [PubMed - indexed for MEDLINE]12: Related Articles, LinksPrawitt D, Brixel L, Spangenberg C, Eshkind L, Heck R, Oesch F, Zabel B, Bockamp E.

RNAi knock-down mice: an emerging technology for post-genomic functional genetics.
Cytogenet Genome Res. 2004;105(2-4):412-21. Review.
PMID: 15237229 [PubMed - indexed for MEDLINE]13: Related Articles, LinksSykes DB, Kamps MP.

Estrogen-regulated conditional oncoproteins: tools to address open questions in normal myeloid cell function, normal myeloid differentiation, and the genetic basis of differentiation arrest in myeloid leukemia.
Leuk Lymphoma. 2003 Jul;44(7):1131-9. Review.
PMID: 12916864 [PubMed - indexed for MEDLINE]14: Related Articles, LinksRiminton DS.

Gene targeting technology and advances in the pathophysiology of inflammation.
Pathology. 2002 Apr;34(2):109-14. Review.
PMID: 12009090 [PubMed - indexed for MEDLINE]15: Related Articles, LinksMüller MB, Keck ME.

Genetically engineered mice for studies of stress-related clinical conditions.
J Psychiatr Res. 2002 Mar-Apr;36(2):53-76. Review.
PMID: 11777495 [PubMed - indexed for MEDLINE]16: Related Articles, LinksBabinet C, Cohen-Tannoudji M.

Genome engineering via homologous recombination in mouse embryonic stem (ES) cells: an amazingly versatile tool for the study of mammalian biology.
An Acad Bras Cienc. 2001 Sep;73(3):365-83. Review. Erratum in: An Acad Bras Cienc 2001 Dec;73(4):577-80.
PMID: 11600898 [PubMed - indexed for MEDLINE]17: Related Articles, LinksCohen-Tannoudji M, Babinet C.

Beyond 'knock-out' mice: new perspectives for the programmed modification of the mammalian genome.
Mol Hum Reprod. 1998 Oct;4(10):929-38. Review.
PMID: 9809673 [PubMed - indexed for MEDLINE]18: Related Articles, LinksJaisser F, Beggah AT.

Transgenic models in renal tubular physiology.
Exp Nephrol. 1998 Sep-Oct;6(5):438-46. Review.
PMID: 9730660 [PubMed - indexed for MEDLINE]19: Related Articles, LinksBernstein A, Breitman M.

Genetic ablation in transgenic mice.
Mol Biol Med. 1989 Dec;6(6):523-30. Review.
PMID: 2699511 [PubMed - indexed for MEDLINE]